Description:

REAL-TIME MULTIPLEX MEASUREMENT OF ANTIBODY-ANTIGEN BINDING CONSTANT USING LAB-ON-CHIP (LOC) DEVICE

INV-14118

INVENTORS: Tania Konry; Noa Cohen; Alexander Golberg

Description       

The detection of soluble molecules such as biomolecules, drugs, antigens, and antibodies (especially the ability to monitor their time-varying concentrations and reaction constants) on a real-time basis is highly challenging.  In prior-art, some of the immunoassay based techniques (including ELISA) for detection of such molecules are already known. However, these techniques are associated with one or more potential limitations such as limited surface area, low sensitivity, slow speed, and lack of automation. This invention relates to the development and use of a novel lab-on-chip (LOC) device for a real-time measurement of an antigen-antibody binding constant, overcoming prior-art limitations and/or addressing unmet needs.

       

Value Proposition

The LOC device:

•Is capable of detecting trace amounts of analytes in an automated manner

•Allows for a faster detection, with sensitivity better than or equal to that of ELISA

•Is based on a microsphere based (bead-based) immunoassay with a microfluidic format

•Uses a larger detection area (surface area), enabling the detection of wide range target analytes

•Uses a mathematical model for effectively calculating the antigen-antibody binding constant on real-time basis

•Effectively allows for a real-time monitoring of clinically relevant target analytes (multiplex analytes) using        antigen-antibody interactions

•Would be commercially useful for the following applications:

•Pharmacokinetic analysis in real time

•Real-time antibody affinity demonstration

•Real-time analysis of multiplex disease markers

•In-vivo detection of analytes on real-time basis

 

Intellectual Property status

Provisional Application 62/050,538

 

License status

Available for license

 

Patent Information:
For Information, Contact:
Mark Saulich
Associate Director of Commercialization
Northeastern University
m.saulich@northeastern.edu
Inventors:
Tania Konry
Noa Cohen
Alexander Golberg
Keywords: