Description:
 
Background
This technology introduces two approaches to polymer sequencing using nanopores in electrically conducting, ion‐intercalating MXene membranes, split into two applications:
- The first application of MXene membranes is based on ion transport localization between an ultrathin nanopore composed of intercalated ions (between MXene sheets), and an electrolyte chamber.
- In the second application, ion transport localization between two ultrathin ion intercalating MXene electrodes provides a finite path for ions to afford true single-base resolution.
 
Technology Overview
- In the first application, the proposed technique sequences DNA/RNA/protein molecules by using solid‐state nanopores composed of intercalating 2D materials as one of the working electrodes.
- In the second application, the proposed technique sequences DNA/RNA/protein molecules by using solid‐state nanopores composed of intercalating 2D materials as working electrodes isolated by an insulator layer in between, and immersed in an electrolyte buffer.
- These designs resolve the problem of access resistance for ions going through atomically-thin pores that considerably affect their sensing resolution.
 
Benefits
- Self‐assembly of two‐dimensional materials to form freestanding membranes using a solvent‐solvent interface method
- Allows for dynamic changes of nanopore thickness 
- Use of synthetic materials instead of polymer‐embedded proteins resulting in higher mechanical stability, durability, and robustness
- Unlike most 2D materials, MXene pores are hydrophilic, which is more biocompatible for biomolecule analysis than most 2D materials
 
Applications
- Long‑read sequencing of DNA/RNA/protein molecules 
- Detection of a greater set of modifications in RNA and proteins
- Structural analysis of DNA, RNA, proteins and other biomolecules
- Long‑read mapping of DNA sequences by sequence‑specific tagging
 
Opportunity
- License
- Partnering
- Research Collaboration
Patent Information:
Category(s):
Biomaterials
For Information, Contact:
Mark Saulich
Associate Director of Commercialization
Northeastern University
m.saulich@northeastern.edu
Inventors:
Meni Wanunu
Mehrnaz Mojtabavi
Armin Vahid Mohammadi
Majid Beidaghi
Keywords:
DNA sequencing
Nanopores
Protein sequencing
RNA sequencing
Single-molecule mapping