Description:
 
Background
Quantifying proteins in limited samples are required for many practical applications, including biopsies, tissue samples, clinical trial of pharmaceuticals, and others. Several methods have been developed for doing sample prep with minimal losses. However, all existing methods uses a chemical that undermines mass-spec analysis or involves expensive equipment. 
 
Technology Overview
In this invention, a novel method to process proteomic applications is performed by a small amount of triethylammonium bicarbonate added to a final concentration, followed by the same volume of trypsin and benzonase. The sample is digested, and then the digestion is quenched with formic acid to a final concentration of 1% v/v. At this point, the sample is analyzed by mass spectrometry. The sample is then incubated at room temperature with shaking for 1 hour. 
The reaction is quenched with a final concentration of Ammonium bicarbonate and incubated for a further 30 minutes. The reaction is further inhibited by adding 1% v/v formic acid to acidify the solution. Samples are combined for multiplexed analysis, dried to appropriate injection volumes if necessary, and analyzed by mass spectrometry without further processing or clean up. 
 
Benefits
- High-throughput processing and automation
- It provides similar or greater lysis yield than other state-of-the art techniques for low-input proteomic samples 
- mPOP is at least 10 times cheaper that Covaris or filter based (FASP, S-Trap) preparations 
 
Applications
- Biomarker discovery
- Pharmaceutical R&D
 
Opportunity
- License
- Partnering
- Research collaboration
Patent Information:
For Information, Contact:
Mark Saulich
Associate Director of Commercialization
Northeastern University
m.saulich@northeastern.edu
Inventors:
Nikolai Slavov
Bogdan Budnik
Harrison Specht
Keywords: