Northeastern researchers propose a novel system for high‑resolution DNA and RNA sequencing, protein identification, and protein conformational change monitoring.
Technology Overview
The proposed invention describes a stable and water-soluble portal protein channel based on the thermophilic bacteriophage G20C, which is demonstrated to be a robust, chemically programmable channel that either voltage- or pressure-inserts into a solid-state nanopore matrix to form a hybrid nanopore sensor device. The signal for sensing using this device can be either electrical or optical, the latter offering a high-density parallelized readout from multiple adjacent pores. Key elements of the innovation include mechanisms to obtain the hybrid structure, stabilize it, and modify it so that different types of biomolecules can be sensed.
- Rapid and stable insertion of unmodified protein into a solid-state nanopore
- Mutations for sensing improvement 
- Translocation of biopolymers (nucleic acids and polypeptides) for sensing applications
- High-resolution mapping of DNA, RNA sequencing, DNA sequencing 
- Protein identification, protein conformational change monitoring 
- Polypeptide sequencing 
- Small-molecule detection, biomolecular complex detection, enzyme-ligand binding 
- Biotechnology and agri-food sectors
- Licensing
- Research collaboration
- Sponsored research
Patent Information:
For Information, Contact:
Mark Saulich
Associate Director of Commercialization
Northeastern University
Meni Wanunu
Alfred Antson
Sandra Greive
Benjamin Cressiot