Metallic nanoparticles (NPs) have been used in the healthcare industry as antioxidants, antimicrobials, and anticancer agents. With the increasing demand, there is a need for eco-friendly, cost-effective, efficient, and consistent production methods of nanoparticles. Recent studies show that NPs can be produced using bacterial cells, lowering the industrial and energy waste in the process. However, the current process requires extensive human labor, which brings viable results between batches, also varying in yield, and risking contamination. 

The current invention puts forward the design of a perfusion bioreactor for the synthesis and recovery of NPs produced by bacterial cultures. 

Technology Overview

The design of a specialized bioreactor is based on a conventional flat-bed bioreactor with compartmentalization into a holding compartment, a reacting compartment, and a collecting compartment, as well as an inline filtering system the, purifies nanoparticles produced by the bacteria. 

The structural design ensures the uniform medium exposure to all cultured cells and the prevention of cell loss due to the washout effect. The design utilizes material whose properties support the adhesion of bacterial cells throughout the exchange of culture medium and the maintenance of a biologically optimal environment. 


  • Consistency in nanoparticles produced 
  • Multiple production lines simultaneously 
  • Adaptable for production of various nanoparticles 
  • Reduction in required resources 
  • Increased efficiency of production 
  • Minimal human interference 
  • Minimal risk of contamination 


  • Bacteria’s cell culture: 
    • Nanoparticles production 
    • Cellulose and other polymers production 
  • Mammalian cells biocomponents production 
  • Yeast’s biocomponents production 


  • License 
  • Partnering 
  • Research collaboration 
Patent Information:
For Information, Contact:
Mark Saulich
Associate Director of Commercialization
Northeastern University
Linh Truong
David Medina Cruz
Thomas Webster
Cell Culture
nanomaterial synthesis