Description:

Single Cell Fluorescence In Situ Hybridization In Microfluidic Droplets

A BREAKTHROUGH PLATFORM FOR PATIENT CUSTOMIZED CANCER IMMUNOTHERAPY

 

Background

 

The goal of Personalized Medicine is to deliver highly specialized healthcare to every patient based on their unique biological characteristic derived from genetic, proteomic, and metabolic information.

It is necessary to devise single cell analysis technologies that allow investigation of every aspect of cellular response. And so, the need for this platform.

Current immunotherapeutic approaches such as vaccination, aim to activate host immune responses and induce disease regression. Efficient activation of the immune system requires cell-cell interaction that can be studied at the single cell level. However, there are few existing methods that allow dynamic analysis of these cell-cell interactions.

 

Technology Overview

This microfluidic droplet array platform permits rapid, functional assessment of various aspects of anti-tumor immunity at single cell resolution. The platform permits analysis of cellular proteins and mRNA simultaneously by using an integrated droplet generation and droplet merging approach. It not only allows for development of personalized treatments, but can also be used as a diagnostic for detection of disease.

 

 

Benefits 

The key benefits of this technology include:

 

  • Direct assessment of specific proteins and their mRNA in single cells.

  • High throughput detection of single cells.

  • Rapid detection as compared with standard FISH methodology.

  • Utilizes simple, inexpensive, and portable instrumentation.

  • Provides ability to customize therapy for each patient.

Applications

  • Assaying clinical oncological samples to detect chromosomal abnormalities like leukemia

  • Correlating protein expression with changes in DNA

  • Adaptable for clinical diagnoses of diseases demonstrating chromosomal alterations as well as with transplant patients

 

Intellectual Property Status

Pending US, EP, CA, AU

 

Development Status/Data

Lab Prototype and data

 

Patent Information:
Category(s):
Biotechnology
For Information, Contact:
Mark Saulich
Associate Director of Commercialization
Northeastern University
m.saulich@northeastern.edu
Inventors:
Tania Konry
Saheli Sarkar
Keywords: