Description:

Title: Antibody-based Rolling Circle Amplification for COVID-19 Detection by a Colorimetry

 

Background

 

Viruses are conventionally detected by RT-PCR. This method requires long analysis time, highly trained professionals, and often shows non-specific characteristics. Antigens and antibodies (Ag/Ab) are commonly used for fluorescence immunoassay. However, the fluorescent signal from the individual fluorescent molecule attached to the antibody is extremely weak and requires signal amplification.

This invention provides a way for a quicker and more efficient method of DNA and virus detection by rolling circle amplification. The integration of the multi-padlock-based RCA approach with Ag/Ab immune-RCA has many advantages over the conventional Ag/Ab immunoassay.

 

Technology Overview

 

RCA colorimetric detection is applied from the DNA fragment attached to the antibody, sensitively capturing the target viruses, such as SARS-CoV-2. The antibody used is specifically sensitive to the target virus. The antibody-attached DNA is used for the template for amplification. The circular DNA is comprised of a target binding complementary sequence to the target viral DNA colorimetric DNA sequence to be amplified for the later RCA reaction. It gets hybridized with the DNA-attached to the antibody capturing the target virus. The colorimetric readout from the amplified DNA having a special structure “G-quadruplex DNAzyme (GQ-DNA)”. This GQ-DNA product gets transformed in the amplified DNA fragment to be a visible readout of the color signal by hydrolysis of color substrate.

 

Benefits

 

o Fully automated system

o Rapid detection method

o Reduced consumption of analytical reagents including molecular detection probes

o Single virus copy number level of detection is expected

o Lightweight, portable device

o Cost-efficient

o Technical knowledge or training is not required to perform the test

 

Applications

 

o Point of care testing

o Rapid and accurate testing of COVID-19

o Analysis of DNA and RNA fragments, proteins, exosomes, etc.

 

Opportunity

 

o License

o Partnering

o Research collaboration

 

Patent Information:
For Information, Contact:
Mark Saulich
Associate Director of Commercialization
Northeastern University
m.saulich@northeastern.edu
Inventors:
Ji Won Lim
Tania Konry
Keywords:
Analytical Chemistry
COVID-19
Detection
Diagnostics