Description:

INV-23089

Background:

Nanopore sensing devices are integral to scientific research and applications such as DNA sequencing and protein detection. The dynamic of trans and cis user access to a membrane in such devices can, however, pose significant challenges. Fulfilling the need for multiplexed and scalable nanopore sensing systems, especially those with both cis and trans access, has been a persistent issue in the field. Existing technologies usually do not support simultaneous user access to both sides of a membrane and lack multi-channel isolation during experiments. This limitation often results in crosstalk and interference between channels, negatively impacting the precision and reliability of readings. Furthermore, many current approaches lack the flexibility to test varying combinations of membranes, pores, and electrolyte solutions, curtailing the potential versatility of nanopore sensing systems.

Technology Overview:

Northeastern researchers have invented a multiplexed nanopore sensing device that incorporates unique design elements to optimize experimental control and scalability. The inventive feature of this technology lies in its capacity for user access to both the cis and trans sides of a membrane. The technology allows different combinations of membranes, pores, and electrolyte solutions to be tested, with a provision for fresh nanopore sensors to replace expired ones. Such a novel architecture sets this technology apart, solving the problem of multi-channel isolation while managing solution flow. It breaks new ground by providing the ability to insulate all recording trans chambers from a master flow canal on demand. As such, it surpasses traditional, limited design nanopore sensing systems by offering greater control, scalability, and versatility.

Benefits:

  • Increased experimental control with each channel's electrical isolation
  • Scalability and capabilities to test a diverse array of membranes, pores, and solutions
  • Improved precision and reliability of readings due to reduced crosstalk and interference
  • Enhanced versatility with replaceable nanopore sensors

Applications:

  • Next-generation DNA sequencing for improved genomic research and precision medicine
  • Detection and analysis of proteins in clinical samples for diagnostic purposes
  • Drug discovery process by studying nanoscale biological processes
  • Environmental monitoring for detection of biohazard substances
  • Food safety testing for harmful bacteria detection

Opportunity:

Seeking licensee/industry partner/funding

 

Patent Information:
For Information, Contact:
Vaibhav Saini
Senior Manager Commercialization
Northeastern University
v.saini@northeastern.edu
Inventors:
Meni Wanunu
Ali Fallahi
Amr Makhamreh
Keywords:
Biosensing
Drug Discovery
Microfluidics
Nanopores
Protein sequencing